Polyethyleneimine-associated polycaprolactone-Superparamagnetic iron oxide nanoparticles as a gene delivery vector. > 연구자료실

본문 바로가기

연구자료실

연구자료실
IP: ***.***.***.4.1

Polyethyleneimine-associated polycaprolactone-Superparamagnetic iron o…

페이지 정보

최고관리자 조회수 : 2,574

본문

J Biomed Mater Res B Appl Biomater. 2017 Jan;105(1):145-154. doi: 10.1002/jbm.b.33519. Epub 2015 Oct 6.

Polyethyleneimine-associated polycaprolactone-Superparamagnetic iron oxide nanoparticles as a gene delivery vector.

Abstract

This study describes the synthesis of novel gene delivery vector with low toxicity and high transfection efficiency for magnetofection. The rational design of magnetofection vector called PPMag (PEI-associated polycaprolactone (PCL)-SPIONs) composed of oleic acid (OA) stabilized superparamagnetic iron oxide nanoparticles (SPPIONs) prepared by thermolysis of iron oleate with a combination of hydrophobic PCL and proton absorbing polymer polyethyleneimine (PEI) (PEI-PCL-SPIONs) is described. Encapsulation of amphiphilic PEI with SPIONs not only improves water dispersity of SPIONs, but also allows nucleic acid (NA) condensation and endosomal/lysosomal escape via proton sponge effect after internalization in cells. MTT cytotoxicity assay showed that cell viability was improved compared to conventional PEI-SPIONs. The luciferase activity of magneto-polyplexes treated cells significantly improved compared to both controls revealed that transfection efficiency of PPMag- pCIKlux polyplexes group was improved compared to naked pCIKlux group. The application underneath of a rare earth magnet significantly improve the transfection efficiency (i.e., the luciferase activity doubles) compared to cells without magnet, indicating that sedimentation induced by magnetic field plays important role in accumulation of magneto-polyplexes on cell surfaces. The results demonstrate that PPMag can be used as a novel gene transfection vector to improve transfection efficiency.

KEYWORDS:

gene delivery; iron oxide; polycaprolactone; polyethyleneimine; superparamagnetic

대표 : 김좌진 ㅣ 개인정보관리 : 김좌진 ㅣ 사업자번호 : 253-86-01081 ㅣ 통신판매업신고: 2018-대전동구-0190호
본사 : 대전광역시 동구 충정로 21, 4105호 ㅣ 기업부설연구소 : 대전광역시 동구 충정로 21, 4104호
당사는 모든 이미지의 무단사용을 금하며, 무단사용시 저작권법 98조에 의거 민형사상 책임을 지게됩니다.
COPYRIGHT ©2019 (주)프로셀컴퍼니. ALL RIGHTS RESERVED.